Plasmid-mediated mineralization of naphthalene, phenanthrene, and anthracene.
نویسندگان
چکیده
The well-characterized plasmid-encoded naphthalene degradation pathway in Pseudomonas putida PpG7(NAH7) was used to investigate the role of the NAH plasmid-encoded pathway in mineralizing phenanthrene and anthracene. Three Pseudomonas strains, designated 5R, DFC49, and DFC50, were recovered from a polynuclear aromatic hydrocarbon-degrading inoculum developed from a manufactured gas plant soil slurry reactor. Plasmids pKA1, pKA2, and pKA3, approximately 100 kb in size, were isolated from these strains and characterized. These plasmids have homologous regions of upper and lower NAH7 plasmid catabolic genes. By conjugation experiments, these plasmids, including NAH7, have been shown to encode the genotype for mineralization of [9-14C]phenanthrene and [U-14C]anthracene, as well as [1-14C]naphthalene. One strain, Pseudomonas fluorescens 5RL, which has the complete lower pathway inactivated by transposon insertion in nahG, accumulated a metabolite from phenanthrene and anthracene degradation. This is the first direct evidence to indicate that the NAH plasmid-encoded catabolic genes are involved in degradation of polynuclear aromatic hydrocarbons other than naphthalene.
منابع مشابه
Polycyclic Aromatic Hydrocarbons Classification of Pahs__________________________ Compound Effect
1. Unsubstituted 6-membered aromatic ring systems (fused, unfused, or mixed) chrysene mutagenic initiator carcinogenic pyrene co-carcinogen mutagenic benzo[a]pyrene mutagenic carcinogenic fetotoxic teratogenic benzo[e]pyrene carcinogenic mutagenic benzo[a]anthracene mutagenic carcinogenic benzo[c]phenanthrene initiator mutagenic naphthalene inhibitor phenanthrene initiator mutagenic anthracene ...
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 59 6 شماره
صفحات -
تاریخ انتشار 1993